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Page 1.

EFFICACY EVALUATION
CB– RSG BIOLOGICAL FERTILIZER

Objectives
Methodology

INTRODUCTION

Fertilizer use is one of the basic considerations in agriculture. It can be soil-applied using inorganic and/or organic fertilizers, directly sprayed to plant leaves with liquid/foliar fertilizers or a combination of both.

The soil-applied fertilizers have relatively higher amounts of macro-elements such as nitrogen, phosphorus and potassium. On the other hand, the very high macronutrient required by most crops cannot be provided by liquid/foliar fertilizers but they normally contain essential microelements required for a balanced plant nutrition.

CB-RSG is one of the many liquid fertilizer formula which contain organic biochemical macro and micronutrients. As a requisite for registration to the FPA, this study was specifically conducted to evaluate the effectiveness of CB-RSG liquid fertilizers on the growth and yield of lowland rice.

OBJECTIVES:

  1. To determine the effects of CB-RSG on the growth and yield of lowland rice.

  2. To determine the economics of using CB-RSG alone or in combination with inorganic fertilizer.

  3. To generate experimental data for FPA registration purposes.

METHODOLOGY:

Site Selection:
The site was within the National Soils and Water Resources Research Center of the Bureau of Soils and Water Management at San Ildefonso, Bulacan. It typifies an irrigated lowland agricultural domain where the soil is characteristically clayey and classified as a vertisol. The specific soil properties of the experimental area were as follows:

pH – 7.2
%O.M. – 1.50
Olsen P – 8 ppm
Hot K – 68 ppm

Land preparation:
After land clearing, the experimental area was plowed and harrowed twice, respectively.

Experimental design and lay-out:
The experiment was laid out using a randomized complete block design. There were eight treatments replicated three times. Each plot measured 4m x 4m. The different treatments were as follows:

T1 - Control
T2 - M-99 (90-30-30, DS)
T3 - 1/3 M-99 (30-10-10)
T4 - 1/3 M-99 + CB-RSG
T5 - M-99 (45-15-15)
T6 - M-99 + CB-RSG
T7 - CB-RSG alone
T8 - M-99 + CB-RSG

Seedbed preparation and transplanting:
A wet seedbed was employed. For treatments using CB-RSG, IR-42 rice seeds were soaked overnight in a solution using 2 cc CB-RSG per 20 liters of water treated with 2 percent Benlate fungicide. For treatments without CB-RSG, seeds were only soaked in 2 percent Benlate fungicide solution. IR-42 is very susceptible to Bakanae and Benlate fungicide proves to be an effective antidote to this as far as our field experiences are concerned.

Inorganic fertilization:
For treatments 2 and 8, one/half of the N fertilizer (Ammonium sulfate) and all of the required P205 and K2O were basally applied. The remaining N fertilizer was topdressed before panicle initiation. For treatments 3, 4, 5, and 6, all the inorganic fertilizers were basally applied.

CB-RSG organic liquid/foliar fertilization:
At the last leveling, the corresponding CB-RSG treated plots were soil-drenched at the rated of 10 cc CB-RSG per 16 liters of water (one knapsack-sprayer load). After 10 days, weekly foliar sprayings (up to six weeks) were done at the rate of 3 cc CB-RSG per knapsack-sprayer load of water. Thereafter, two or more additional foliar sprayings using the same rate were done at 15 days interval.

Transplanting:
The seedlings were transplanted in straight rows at the rate of 3 seedlings per hill 21 days after sowing and following the basal application of granular fertilizers and soil-drenching with CB-RSG to corresponding plots. Missing hills were replanted after 5 days.

Pre-harvest data gathering:
1. Mean plant height at maximum tillering stage (60 DAS or before panicle initiation).

2. The average plant height, in cms, for each treatment was determined through individual height measurements taken from the base to the height of the tallest leaf of plants from the 4 hills in the 4 corners of each plot (a total of 16 hills).

3. Tiller count at maximum tillering stage (60 DAS).

From the same 16 hills, tiller counts were recorded and transformed to the number of tillers per m2. The area covered by the 16 hills was 0.64 m2.

Harvesting:
A total of 100 hills per plot were harvested. This covered a harvest area of 4 m2.

Data gathering of yield and yield components at harvest:
The missing data technique of statistical analysis was employed for straw weight and grain yield because there were taken from the 4 m2 harvest area of Rep 1 – T2, T3 and T6 plots which were 50 percent rat-damaged during harvest.

For the other yield components such as plant height, panicle count and fresh weight of panicles, the data were collected from the 4 corner hills of each plot (16 hills) which were not rat-damaged. The 16 hills cover an area of 0.64 m2. To round up, this was transformed to 1 m2.

Mean plant height:
From the tagged 16 hills, measurements in cms. were done from the base to the height of the tallest panicle. These measurements were then averaged.

Panicle count:
The panicles from each tagged 16 hills were counted and this count represented the productive tillers per plot. Each count was likewise transformed into panicle count per m2.

Fresh weight of panicles, in gms:
The counted panicles per plot were weighed in gms and this was also transformed to per m2 weight.

Straw weight, in kg:
The 100 hills of rice plant from the 4 m2 harvest area were threshed and straws were weighed.

Grain yield in tons/ha:
The threshed grains were cleaned, winnowed, dried for 3 days and weighed. Each yield from 4 m2 harvest area was transformed into tons per ha.

Economic analysis:
The cost and return of using CB-RSG organic liquid/foliar fertilizer, alone or in combination with organic liquid/foliar fertilizers, was analyzed. A comparative analysis with that of the M-99 recommendation for dry season was also done.

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